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Maxi-prep kits are not working - why?

Maxi-prep kits are not working - why?

Science

z

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03 Oct 05
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86698
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23 Apr 09
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At first I thought it was just me. But, others are having the same problem. Despite the fact that the methods have not changed and we buy (as far as we know) the same kits as always, today's maxi-prep kits are yielding less and less plasmid DNA.

WHY?!!!!

AThousandYoung
1st Dan TKD Kukkiwon

tinyurl.com/2te6yzdu

Joined
23 Aug 04
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26753
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28 Apr 09
2 edits
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Global warming.

All steps should be performed chilled (on ice) except for the phenol/chloroform step(s) and the RNase incubation step (obviously!).

http://openwetware.org/wiki/Streptomyces: Protocols/Mini-Maxi_Prep


EDIT - Space added to address to avoid smiley.

z

Joined
03 Oct 05
Moves
86698
Clock
28 Apr 09
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Originally posted by AThousandYoung
Global warming.

All steps should be performed chilled (on ice) except for the phenol/chloroform step(s) and the RNase incubation step (obviously!).

http://openwetware.org/wiki/Streptomyces: Protocols/Mini-Maxi_Prep


EDIT - Space added to address to avoid smiley.
Interesting idea!

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