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Science Forum

Science Forum

  1. 23 Apr '09 23:59
    At first I thought it was just me. But, others are having the same problem. Despite the fact that the methods have not changed and we buy (as far as we know) the same kits as always, today's maxi-prep kits are yielding less and less plasmid DNA.

    WHY?!!!!
  2. Subscriber AThousandYoung
    155 years
    28 Apr '09 16:03 / 2 edits
    Global warming.

    All steps should be performed chilled (on ice) except for the phenol/chloroform step(s) and the RNase incubation step (obviously!).

    http://openwetware.org/wiki/Streptomyces: Protocols/Mini-Maxi_Prep


    EDIT - Space added to address to avoid smiley.
  3. 28 Apr '09 21:16
    Originally posted by AThousandYoung
    Global warming.

    All steps should be performed chilled (on ice) except for the phenol/chloroform step(s) and the RNase incubation step (obviously!).

    http://openwetware.org/wiki/Streptomyces: Protocols/Mini-Maxi_Prep


    EDIT - Space added to address to avoid smiley.
    Interesting idea!