1. Joined
    03 Oct '05
    Moves
    86698
    23 Apr '09 23:59
    At first I thought it was just me. But, others are having the same problem. Despite the fact that the methods have not changed and we buy (as far as we know) the same kits as always, today's maxi-prep kits are yielding less and less plasmid DNA.

    WHY?!!!!
  2. SubscriberAThousandYoung
    West Coast Rioter
    tinyurl.com/y7loem9q
    Joined
    23 Aug '04
    Moves
    24791
    28 Apr '09 16:032 edits
    Global warming.

    All steps should be performed chilled (on ice) except for the phenol/chloroform step(s) and the RNase incubation step (obviously!).

    http://openwetware.org/wiki/Streptomyces: Protocols/Mini-Maxi_Prep


    EDIT - Space added to address to avoid smiley.
  3. Joined
    03 Oct '05
    Moves
    86698
    28 Apr '09 21:16
    Originally posted by AThousandYoung
    Global warming.

    All steps should be performed chilled (on ice) except for the phenol/chloroform step(s) and the RNase incubation step (obviously!).

    http://openwetware.org/wiki/Streptomyces: Protocols/Mini-Maxi_Prep


    EDIT - Space added to address to avoid smiley.
    Interesting idea!